FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_310_rr.351000000a9603.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_310_rr.351000000a9603.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358753
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACTGTACCCAATGTGTAGTCTAGACAAAGATTTTAAATCAACTA26220.1111604309565266No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT11250.075.4011465
TAACCGA300.001936115872.499565145
GTAGTAA27900.063.672591
ACGCACT15200.057.71474
TAGTAAC31350.055.9669462
GCGGCGT450.00964097548.3330469
AGCACGC18850.044.236131
GTAACAA39750.043.9566464
AACACGA15300.041.699986
GCACGCA20700.041.3300062
TAACAAG42800.040.824225
AGTAACA44550.039.8715363
AAGAGCG13900.039.118479
ACACGAG18600.033.9110877
AAACACG21650.033.822685
CACGAGC19500.032.345968
GTAGAAA43450.032.207591
AAGCGGT5800.031.2498137
CGAGCTA1204.0593412E-430.2087945
CACGCAC29800.029.4383663
Produced by FastQC (version 0.11.2)