FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_306_rr.351000000a95e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_306_rr.351000000a95e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597833
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA49120.1890806683878448No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC49050.18881121303794354No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA48900.1882338087167266No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT46950.1807275525409062No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG42340.16298199306883854No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT40910.15747740520657025No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG38300.14743057001739526No Hit
CTATAGAACATGCTGGTGAATTCAAACATTCCAGTTTCGTTGCAGTAACT38170.14693015293900724No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT33590.12930007433118296No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC33580.12926158070976848No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT33510.12899212535986726No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG31420.12094695848424437No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG29640.11409509387246985No Hit
GTAGAAACACGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA29090.11197794469467437No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT28840.11101560415931278No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA27940.10755117823201107No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC27850.1072047356392809No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA26500.10200809674832832No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACTAAT26260.1010842498343812No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCAGCTGG26030.10019889654184852No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAGG100.0070913667145.000986
ACGAGCG100.0070917746144.998189
CGGTGCA5700.0108.114762
AGCACGC1150.0107.1746441
CACGCAC1300.0100.385293
GCACGCA1250.098.600662
GAAACAC15250.098.41054
GGTGCAT6450.095.543293
AAACACG15950.094.546095
CGCACTT1350.091.296915
AACACGT1350.085.9265066
ACGCACT1450.085.000574
ACACGTG1500.077.333867
GTAACAA47050.077.20034
GCGGAGC9600.075.521351
ACGTGCA2150.074.1865542
AACACGA19200.072.87816
GCGGTGC14800.067.1119461
GCGGAGG451.19417615E-464.4448851
ACACGAG21850.064.039347
Produced by FastQC (version 0.11.2)