FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_302_rr.351000000a95ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_302_rr.351000000a95ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517919
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT5900.072.4993065
GTAGTAA20400.061.8376461
TAGTAAC20350.060.208272
AGCACGC7400.058.7832221
ACGCACT7800.054.8392144
GTAACAA23700.051.697824
AACACGA10950.049.6570636
ACGAGCG1351.9099389E-1048.332879
TAACAAG26450.046.322815
GCACGCA9900.043.2066572
ACACGAG12800.041.9136627
TAGACCG700.001056863841.4281775
CACGAGC13700.039.1602138
AAACACG15200.038.1575285
AGTAACA32200.038.050883
CGAGCGA1001.3918665E-436.2496577
ACGAGCA6700.035.7086149
GAAGCGG6150.035.3655176
AAGCGGT4850.032.8862847
AAGAGCG9300.031.1824979
Produced by FastQC (version 0.11.2)