FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_297_rr.351000000a95a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_297_rr.351000000a95a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141451
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC55460.25898327816046224No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT52290.2441802310676266No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA48810.22792956738211614No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT39600.18492134538684285No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT30690.14331404267480322No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA30320.1415862422254817No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT29850.13939146868174895No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA29390.13724339244745734No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG26440.1234676861623264No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT25880.12085263683362356No Hit
CCCCATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG25800.12047905835809458No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT25180.11758382517274503No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG25090.11716354938777493No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG24470.11426831620242536No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG24230.11314758077583843No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT23840.11132638570763469No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC23070.10773069288066829No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT22980.10731041709569819No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT22810.10651656283519911No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA22670.10586280050302341No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG22470.10492885431420099No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC22370.10446188121978976No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGT21700.10133316148723459No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG100.007090021145.00961
CGACACG100.007091011145.002825
TCGCAGG100.007091506144.99944145
CACGCGC100.007091506144.999449
CGGTGCA2250.0103.1155242
GAAACAC13250.0101.7755664
AAACACG13650.098.261995
AGCACGC1150.094.571471
ACGCACT1300.083.655484
CGCACTT1300.083.655485
GTAACAA38750.081.7628864
GCACGCA1400.077.68192
GGTGCAT3250.073.618543
CAGCCTT4100.072.501415
AACACGA17900.072.501416
TAGTAAC47750.067.264662
ACAAGAG48550.065.705217
ATACCGA451.1937772E-464.448711
GCGGAGC7050.063.7630841
GTAGTAA52100.063.04161
Produced by FastQC (version 0.11.2)