FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_291_rr.351000000a958b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_291_rr.351000000a958b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2004061
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG9000.058.002465
GTAGTAA17550.051.640361
AACACGA9250.049.3804666
ACACGAG9450.048.3353847
CACGAGC9500.048.0809828
TAGTAAC18400.046.890572
CTGCGCG1801.8189894E-1244.30522145
CGCACTT4000.036.2515345
ACGCACT4150.034.9412354
ACGAGCA5850.034.7005929
AGTAACA27350.032.60653
TAACAAG28100.031.47825
TACGACC950.00472743830.5276098
GTAACAA29450.030.5276094
GCACGCA6200.025.7268962
ACGAGCC6000.025.3748079
AGCACGC6100.024.9600731
CAAGAGC35700.023.9646028
GTAGAAA43900.023.2868611
TAGAAAC41350.023.1448732
Produced by FastQC (version 0.11.2)