FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_286_rr.351000000a9561.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_286_rr.351000000a9561.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474295
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCG1050.069.046689
AACACGA4350.058.3365026
GCGTACA805.873426E-754.3779532
CGGTGCG450.00964055348.332687
GTAGTAA15200.047.706431
TAGTAAC16250.045.0639842
TAACAAG16550.044.6852045
CGCACTT3150.041.430825
GCACGCA4200.039.7045362
CGTACAC1105.2640407E-639.54763
AAGAGCG6650.039.247599
AAACACG8550.039.007965
GTAACAA18500.038.7994044
ACACGAG6850.037.0432937
TAGTAGT6500.031.2324644
CACGAGC8550.029.6779588
AAGCGGT3251.8189894E-1229.0015745
ACGAGCC2502.4501787E-928.9996059
CTAGCTA3403.6379788E-1227.7202139
AGTAACA27250.027.139093
Produced by FastQC (version 0.11.2)