FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_285_rr.351000000a9555.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_285_rr.351000000a9555.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518999
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC48690.1932910652207484No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT47790.18971821743478262No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT46380.18412075590343624No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT43730.1736007040892037No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT40330.16010327911999964No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC38230.1517666342860795No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC37770.14994051208436365No Hit
CCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT36870.1463676642983979No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT36750.14589128459360245No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT34840.13830890762560843No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT33380.13251295455059728No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT32820.1302898492615519No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT32060.1272727777978475No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC31740.12600243191839297No Hit
GTGGAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTT30620.12155622134030224No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA30610.12151652303156928No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC28280.11226681709679122No Hit
AGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAAT28100.11155224753959807No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG28080.11147285092213217No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC27680.10988491857281404No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG26340.10456534520259834No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC26020.10329499932314383No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC25840.10258042976595068No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC2000.0116.022161
GTAACAA34700.0115.964254
CGGTGCA5150.0111.2193762
GAAACAC10950.0103.293054
GAGCAGA4950.0101.079911
CAGCGTT301.5995203E-596.67175
ACGCACT2550.093.828414
GCACGCA2500.092.8048252
AAACACG12150.092.494525
TAACAAG43850.092.26255
CGCACTT2650.090.287725
TTGCGCC259.388696E-486.999344145
CGGAGCG1550.079.520262
TAGAAAC15450.079.3083342
GGAGCGT1600.077.0352553
TAGTAGA2450.073.9834443
AACACGA14100.073.5321966
GCGGAGC8500.072.513851
CAGCATC5100.072.503774
CACAGCG406.663433E-572.503773
Produced by FastQC (version 0.11.2)