FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_284_rr.351000000a953b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_284_rr.351000000a953b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219745
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT37790.17024478036891624No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG33870.15258509423379712No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC33310.1500622819287801No Hit
CTATAGAACATGCTGGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT29970.1350155085381429No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC27680.1246990082194126No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA27090.12204104525519824No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT26210.1180766259187429No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG24420.11001263658663495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT1400.093.2178655
AGCACGC1650.092.286671
CGGTGCA2450.062.1466452
ACGCACT2800.054.3783154
AACACGA8650.053.6436776
AGAAGCG6950.051.117075
ACACGAG9150.050.710047
GAGCGTT1152.4865585E-950.434457
CACGAGC9200.048.858378
AAACACG10650.048.335195
GAAACAC11350.045.3551834
AAGCGGT5750.042.8692787
AGCGGTG5800.042.4997188
GAAGCGG8200.042.440656
GTGTAAA14600.036.2554741
ACGAGCC3700.035.270049
TATAGAG19650.033.9453245
GCACGCA4600.033.0998422
ACACGTG1551.4661509E-632.7417147
TAGATGT12950.032.473024
Produced by FastQC (version 0.11.2)