FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_282_rr.351000000a94f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_282_rr.351000000a94f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498375
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT78290.22378961660771074No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA68110.19469039196769927No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT59000.16864973023189336No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT58090.1660485225283167No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT52720.15069853860721047No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA50310.14380962589773824No Hit
GTATGCAGAAACCAACCTGCTGCAACGGCACTGAATACAACAATAACCTC49760.14223746739557652No Hit
ATTAGTAGCAGATGGTGGGCCCAACATTTACAATTTGAGAAACTTGCATA43440.12417193697073642No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT43110.12322864186943938No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG42990.12288562546896772No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC41620.11896952156358309No Hit
GTGTAGCATTTCTATGAACTCAGCTGATGTTGCTCCTGCCACTGGCAAGA40490.11573945045914175No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGA40410.11551077285882731No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC39050.11162325365348198No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT37740.10787865794833315No Hit
GTGTTTAGTATAGATCTGTTTCTTTTGGTTCTCCAACTATGAGTTCCAGA37700.10776431914817594No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA37390.10687819344695751No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA37330.10670668524672171No Hit
TTCTAATGGTATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC36950.10562046664522814No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA36300.10376246114267339No Hit
GTATTAGTAGTTGAAAACTTGGCTAAAAATATATGTGAAAATCTAGAACA35140.10044663593811412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAA100.007091817144.99861145
GAGCAGA4650.0120.0611951
CTATAGC203.8670204E-4108.748955145
ACACGTG1450.085.0004047
CACGCAC353.4376673E-582.861093
CGCACTT353.437911E-582.859915
AAACACG11250.081.2027055
CACGAGC10250.077.097928
AACACGA10350.076.35526
AACACGT1650.074.6994556
ACGAGCC5450.074.49479
GAAACAC12450.073.3759464
ACACGAG10800.073.1716467
TAGAAAC13650.069.582072
AGCACGC451.19390796E-464.448431
ACGTGCC902.8194336E-1064.443839
AGGCGGG350.00356738362.14227145
AGAAGCG6550.061.9867975
CACGTGC2000.061.6252948
GTAACAA13700.061.388914
Produced by FastQC (version 0.11.2)