FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_280_rr.351000000a94dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_280_rr.351000000a94dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3351980
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC60270.17980417544257424No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG57610.17186856723488803No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC51890.15480402627700643No Hit
GTATGTAGAAACCAGCCTGCTGCAACAGCACTAAACACAACAATAACCTC50160.1496428976306541No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT45610.13606883095961192No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA42480.1267310664144774No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA40960.12219643315294244No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT40930.12210693381225425No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG40650.12127160663249781No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT40410.12055561190699228No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG40190.11989928340861222No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA39500.11784079857278386No Hit
ATGTTAATCCATCCCCTGTGGCCCACTTGGCATAATTGGTTTTCTGTAAT38980.11628947666752187No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT37620.11223217322299059No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT37260.1111581811347323No Hit
CCCATACACCGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA35940.10722021014445195No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA34200.1020292483845369No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT33880.10107458875052953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC1600.095.1642151
AAACACG14350.085.38665
CGGTGCA5900.079.877182
GCACGCA2050.074.272292
AACACGA15000.073.952886
GAAACAC16700.073.3711244
ACACGAG16300.068.053797
ACGCACT2400.063.4399724
CACGAGC18550.060.1901258
CACGCAC2650.057.455933
CGCACTT2400.057.398075
GCGGAGC8800.056.8513531
ACGAGCC8750.056.3425339
GGTGCAT8500.054.5911833
GTAACAA50050.052.1498834
AGAAGCG9200.049.6486785
GCACAGG5750.047.9156272
AACACGT2600.047.4056976
TAACAAG56350.046.834125
AAGCGGT8450.045.474477
Produced by FastQC (version 0.11.2)