FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_279_rr.351000000a94b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_279_rr.351000000a94b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3010207
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT68040.2260309673055707No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA56170.1865984631621679No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT55360.18390761831329208No Hit
GTATGTAGAAACCAGCCTGCTGCAACAGCACTAAACACAACAATAACCTC51130.16985542854694044No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC51070.169656106706283No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG45600.151484598899677No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG41100.13653546085036677No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG39310.13058902593741895No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT39130.12999106041544653No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT33920.11268328058502289No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA33240.11042429972423823No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGCTTTTACTGGTAGCGTTAGGGCA32750.10879650469220223No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC32480.1078995564092436No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA32130.10673684567207503No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT30970.10288329008603062No Hit
GATTATAGATATACCTACAAATGCCACAGGGGAGATTCCAAAGTGGAAGG30570.10155447781498082No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT30310.10069074983879846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA4750.097.697732
AAACACG11550.088.517275
GCACGCA1350.085.937822
GAAACAC12500.081.789964
CGCACTT1250.081.2098855
AGCACGC1400.077.685451
CACGCAC1550.074.849073
GGTGCAT6300.073.660993
ATACCGA300.001935441972.506421
AACACGA13350.072.237266
ACGCACT1550.070.169844
ACACGTG1100.065.9116447
ACACGAG14800.065.644437
TAGTAGA3800.062.9692423
GCGGAGC7250.061.00541
AGAAGCG7600.060.1060075
GAGCAGA11050.058.3988341
CAGGATT2750.058.0070655
CGCACCC502.0110073E-457.999355145
CACGAGC16700.057.7417568
Produced by FastQC (version 0.11.2)