FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_274_rr.351000000a9499.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_274_rr.351000000a9499.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771271
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT45620.16461760686702961No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA34550.12467203676580169No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC34080.12297606405147674No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT33970.12257913426727304No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC33620.1213161758629885No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG33480.1208109925012747No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG32220.11626434224585036No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT30950.11168160746458936No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC30670.11067124074116172No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT30230.10908352160434688No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT29410.10612459048573741No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC28960.10450078682308586No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA28510.10287698316043432No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT28420.10255222242790403No Hit
GTGCTTGTGATTCTAATTCACTCACGTCATTAGCTCCCATTATGACTTCC28400.10248005337623062No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC28000.10103667234276258No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA27890.10063974255855887No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA5200.097.600942
GTAACAA25550.089.386334
AAACACG10700.086.054075
CAGGATT5850.083.0367665
GAAACAC11200.082.212374
GAGCAGA8150.081.845981
AGCACGC1200.078.5469361
CGGAGCG651.5643309E-978.080752
GCACAGG6550.075.270862
CACAGGA7050.069.932513
GCGGAGC5500.069.868321
ACGCACT1350.069.816984
TAGTAGA3550.069.4400253
AACACGA12150.068.0267946
TAGAAAC14950.067.411332
GCACGCA1300.066.926352
GAGCGTT2250.064.446444
AGCGTTT2500.063.801975
CGGAGCA4350.063.3364372
ACACGAG13150.062.3000647
Produced by FastQC (version 0.11.2)