FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_264_rr.351000000a947f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_264_rr.351000000a947f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856787
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT29910.1610847124629804No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT29790.1604384347800798No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC26220.14121167371378623No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG25100.13517974867338042No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT24250.13060194841950098No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG24060.129578675421575No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG23760.12796298121432345No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT22730.12241576443609309No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA22240.11977679723091554No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC21700.11686854765786275No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG19490.10496626699777628No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19210.10345828573767482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA4600.0110.33362
CGCACTT3800.089.677165
AGCACGC3350.086.570731
GAAACAC10700.084.024424
GTAACAA31800.078.661024
AAACACG11450.078.520645
GCGGTGC9600.076.279191
GCACGCA3900.074.3640442
GGTGCAT6850.074.092643
ACGCACT4800.072.5049444
GCGGAGC6700.070.338721
CGGAGCG651.398057E-766.9276352
CGAGCTT554.061332E-665.913585
TAGTAAC40700.062.528832
AACACGA13650.062.1470956
TAACAAG41750.059.7405975
ACACGAG15500.054.7265937
GTAGTAA48700.053.893391
AGTAACA48150.052.854073
AGCGTTT2250.051.5590675
Produced by FastQC (version 0.11.2)