FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_249_rr.351000000a9456.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_249_rr.351000000a9456.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2806413
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT43370.15453890785141033No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT36620.1304868527903769No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT36300.12934660721711308No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT34820.12407297144076798No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC34400.12257639912585923No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC33260.11851427427110692No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG32590.11612688510208584No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC32520.11587745638293437No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC32170.11463031278717709No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA31130.1109245146740697No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT30980.11039002456160231No Hit
CCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT29790.10614973633602752No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA30200.0106.354934
GAGCAGA5050.0106.24511
CGGTGCA5550.0103.203632
GAAACAC12950.096.29854
AAACACG13400.092.523525
CGCACTT703.092282E-1182.8614965
TAGAAAC16350.080.264152
AACACGA14550.079.729276
GAGCATC10700.073.181424
CGGAGCA5600.071.20912
ACACGAG16450.070.516667
TAACAAG45950.069.7425165
TAGTAGA3400.068.2388843
GGAGCAT6000.066.461833
TAGTAAC49850.065.304342
ACGTGCA2000.065.253432
AGCACGC902.8194336E-1064.448981
GCGGAGC7350.063.1336981
AGCATCT13650.062.6772885
CGCCTCC350.003566431862.1461226
Produced by FastQC (version 0.11.2)