FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_137_rr.351000000a943c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_137_rr.351000000a943c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834984
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA63350.22345805126237042No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT52190.18409274972980447No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT41110.1450096367386906No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA38730.13661452763049103No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAACCTCATTTAGGTTGAA36730.12955981409418887No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC36520.12881906917287717No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC36390.12836051279301752No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC34740.12254037412556826No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA33320.11753152751479373No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG32980.11633222621362235No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG32530.11474491566795439No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT31820.11224049236256711No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA30870.10888950343282361No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA30290.10684363650729597No Hit
ATTATGGAGCTGTTAGCTATTATCGTTTTGTGAAACAATATGTTATCATC29460.1039159303897306No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT29180.1029282704946483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT3000.070.0876855
AAACACG10700.069.116455
AGCACGC3050.068.9399261
ACGCACT3200.067.972984
AACACGA10250.065.784586
GAAACAC12000.062.2330324
ACACGAG11150.061.12387
CACGAGC11550.059.0069548
CGGTGCA4400.057.6760752
GCACGCA4200.050.06442
AAGCGGT4300.048.8975267
ACGAGCC5050.048.811479
AGAAGCG5550.048.3363385
ACGAGCT1850.047.0266279
TAACAAG42600.046.8045545
TAGTAAC47750.042.517232
GAAGCGG6400.041.9166686
GTAACAA47300.041.847214
GCGGTGC8950.041.3161431
GTAGTAA56450.037.8905331
Produced by FastQC (version 0.11.2)