FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_100_rr.351000000a9412.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_100_rr.351000000a9412.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390720
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTCCCTCAAGCACCGTTATAACTCTGGAGGTCTATCTGTTA28550.11942009101860526No Hit
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT25370.10611865881408113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC45500.0108.039362
GTAGTAA45950.0107.619211
CGCACTT21000.0107.373155
GTAACAA53750.091.4546664
ACGCACT27800.082.152424
TAACAAG60650.081.528275
AGCACGC27350.078.20881
AGTAACA72800.068.420943
GCACGCA31950.067.3983462
AAGAGCG12350.065.748759
ACAAGAG83200.059.603157
CAAGAGC90400.052.3698358
AACAAGA99200.049.8456546
GTAGAAA41250.047.6361121
AACACGA31750.046.816731
TCTCGCT9400.045.508012
CGAGCTA5450.045.2310875
CTCGCTC9800.043.6505433
TCGCTCT10050.042.5638164
CACGCAC53350.041.1786273
Produced by FastQC (version 0.11.2)