FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs9_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs9_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9074890
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT435270.4796421774809391TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG69050.038.6457411
ACACGTC70550.037.85525513
GATCGGA70150.037.5855031
CACACGT72200.037.05108312
ACGTCTG72350.036.76141715
CACGTCT72600.036.7560414
CAGTCAC73650.036.1127427
CGTCTGA73700.036.08804316
AGCACAC75150.035.74301510
GAGCACA76250.035.314329
AGAGCAC77750.034.7746858
CCAGTCA76350.034.691626
CACATGT76800.034.5456231
TCGGAAG79350.034.3017163
TCCAGTC77100.034.2970725
TCACATG77700.034.17360330
AGTCACA78200.034.0957628
GTCACAT79200.033.77636329
GAACTCC78300.033.71525221
ACATGTC79850.033.39158232
Produced by FastQC (version 0.11.2)