FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs8_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs8_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7171452
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT101580.14164495558221682TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG15800.031.46697811
CACACGT18100.027.71149412
ACACGTC18250.027.4837313
GATCGGA17900.027.294191
ACGTCTG18600.026.96656215
CACGTCT19350.025.46658714
AGCACAC19900.025.31547710
CGTCTGA19850.025.04676416
AGTTCCG20200.024.06959734
CCGTATC20700.022.85083438
GAGCACA22150.022.8432459
GTTCCGT21450.022.66709735
TATCTCG20950.022.367541
CAGTCAC22100.021.99903727
CTCGTAT21650.021.94823344
AGAGCAC23600.021.8127568
TCCAGTC22550.021.65758925
GAACTCC23600.021.34652321
GTCACAG23500.021.06305729
TCTCGTA22450.020.77443743
Produced by FastQC (version 0.11.2)