FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs7_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs7_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5953428
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT170360.2861544642851144TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22600.035.1400911
ACACGTC24300.032.68173213
CACACGT24400.032.3674712
CACGTCT24450.032.3012814
ACGTCTG24600.032.01489315
GATCGGA24500.031.4388471
AGCACAC26550.030.40924610
CGTCTGA26250.030.08633216
CACAGTC26550.029.41518631
CAGTCAC26900.029.19577827
TCCAGTC26850.029.00434925
GAGCACA28050.028.3125139
CCAGTCA27850.028.19987126
GTCACAG28200.027.92788329
AGTCACA28100.027.87069528
GAACTCC28850.027.60367421
CTCGTAT27150.026.98249844
ATCTCGT27200.026.60937942
ACTCCAG30000.026.3988723
AGAGCAC30450.026.1532348
Produced by FastQC (version 0.11.2)