FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs6_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs6_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6304641
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG11600.029.77448811
ACACGTC14150.024.8751813
GATCGGA13550.024.6885991
CACACGT14800.023.63404312
ACGTCTG15300.023.5806115
CGTCTGA15950.023.17134516
CACGTCT15550.022.91855614
AGCACAC16100.021.72570610
GAACTCC16350.021.25895721
CAGTCAC16650.021.0080427
CGTATGC16850.020.88924444
TCCAGTC17550.020.43210425
GAGCACA17200.020.208379
AGTCACC17900.019.78679728
GTCACCT19100.019.35004629
CCAGTCA18650.019.22699726
AGAGCAC18700.019.0579458
TCGGAAG18850.019.0269183
TCGTATG19800.017.9991743
TCTCGTA18450.017.5284841
Produced by FastQC (version 0.11.2)