FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs5_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs5_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6650264
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC72100.10841674856817715TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG14000.025.92780111
CACGTCT14900.024.95209114
ACACGTC15400.023.99910513
ACGTCTG15450.023.77904915
CATCTCG13300.023.32349239
GATCGGA15200.023.3112281
CACACGT15700.023.26045612
CGTCTGA17800.021.13404516
CAGTCAC17900.020.40147427
AGCACAC18500.020.096710
TCCAGTC19100.019.69560225
CCAGTCA19600.019.30540326
GAACTCC19350.018.98637421
GAGCACA20050.018.872549
CGTATGC18150.018.6661144
AGTCACC19900.018.12997828
TCGGAAG20700.018.0695423
TCGTATG18600.017.50498843
ACTCCAG21750.017.09361823
AGAGCAC21800.016.953888
Produced by FastQC (version 0.11.2)