FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs4_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs4_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5994466
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC7750.022.99227529
GCACACG8550.021.35554511
TCACGCC8050.021.31594730
AGTCACG8650.020.85433628
ACACGTC10000.018.03900113
CACACGT10300.017.72717512
ACGTCTG10700.017.27007315
GATCGGA10800.017.115731
CACGTCT10950.017.07668114
CGTCTGA11000.016.99905816
CACGCCA10400.016.7113131
TATCTCG11400.016.4047939
CAGTCAC11750.015.53969527
CGTATGC12500.014.78330444
AGCACAC13000.014.21459810
TCGTATG13050.013.99167943
GAGCACA13000.013.8763879
CTCGTAT13200.013.83314442
TCCAGTC13600.013.58759625
GAACTCC13300.013.56327221
Produced by FastQC (version 0.11.2)