FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs3_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs3_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6889082
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC423200.6143053602787716TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG65650.039.81020711
ACACGTC67250.038.8957613
CACACGT67400.038.8418412
GATCGGA65850.038.8005031
GTCACAC67100.038.55621329
ACGTCTG67950.038.33291215
CACGTCT68200.038.32142614
CGTCTGA68300.038.16868616
CAGTCAC69800.037.31692527
CACACAG69700.037.24421331
GAGCACA70650.037.1796159
CCAGTCA70250.036.98393626
AGCACAC70850.036.9815110
CGTATGC68250.036.9717844
TCCAGTC70300.036.9263425
GATCTCG56150.036.79096239
GAACTCC70950.036.58804321
CACAGTG71200.036.36793533
AGTCACA71500.036.33736428
TCGGAAG73200.036.1585853
Produced by FastQC (version 0.11.2)