FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs2_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs2_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7020863
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC70980.10109868259785158TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG12000.028.5986511
ACACGTC13450.025.84264413
CACACGT14000.024.67026312
ACGTCTG14300.024.15270415
CACGTCT14500.024.12314
ACTGACC14600.023.80777432
GATCGGA14850.022.9715291
CAGTCAC15550.022.35280227
AGCACAC16000.021.72397210
CGTCTGA16400.021.1941216
CGTATGC16300.020.51451344
GAACTCC17150.020.13913321
GAGCACA17800.019.7743479
TCCAGTC18850.018.55628625
TCGGAAG19100.018.2017233
CTGACCA19250.018.05693233
CCAGTCA19500.017.93774226
CTCGTAT18200.017.88977442
AGTCACT19550.017.77921128
AGAGCAC20500.017.3846688
Produced by FastQC (version 0.11.2)