FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs24_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs24_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8222677
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT475670.5784855710615898TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG57950.039.74666611
ACGTCTG59850.038.48486715
GATCGGA58450.038.3682021
ACACGTC60800.037.9197213
CACACGT60800.037.84735512
CGTCTGA61350.037.4363416
CACGTCT63200.036.47973314
AGCACAC63500.036.238110
TATCTCG60650.036.12909741
GAGCACA64350.035.8619889
CAGTCAC64200.035.80871227
TCCAGTC64600.035.6210425
AGAGCAC65150.035.5566948
CTCGTAT63100.035.00395244
CCAGTCA65900.034.91834626
TCGGAAG66750.034.6097343
AGTCACA66300.034.6081428
GTCACAT66550.034.51118529
TCTCGTA64550.033.9790843
ATCTCGT64650.033.8586742
Produced by FastQC (version 0.11.2)