FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs23_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs23_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7788437
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG12450.017.84672411
GTCACAC12900.017.394729
GATCGGA13250.016.44241
ACACGTC13700.016.3789513
ACGTCTG13950.016.0854215
CGTCTGA14600.015.36928916
CACACGT15000.014.95944212
CAGTCAC15700.014.85294727
CACGTCT15650.014.75983214
AGCACAC16500.013.59949210
AGTCACA17150.013.59715928
TCCAGTC17800.013.10063525
CACACTG17150.013.08405931
TATCTCG16650.012.94929741
GAGCACA18000.012.7107179
CTCGTAT17750.012.51808444
GAACTCC18900.012.4545621
CCAGTCA19450.012.328588526
AGAGCAC18400.012.1953538
TCACACT19350.012.16492130
Produced by FastQC (version 0.11.2)