FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs22_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs22_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7506989
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT201720.2687095984821611TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG26500.034.86638328
GTCACGA26900.034.34815229
CACGAGT26750.034.29449531
GCACACG27300.034.08640311
CGAGTGG27750.032.98026333
ACGAGTG28050.032.62709832
ACGTCTG28800.032.5402315
GATCGGA28800.032.1711581
ACACGTC29750.031.5011313
TCACGAG29000.031.48157730
CACACGT30100.031.0617512
CACGTCT30400.030.68285614
CGTCTGA30750.030.47670416
AGCACAC31800.029.81629210
CAGTCAC31100.029.78003327
TATCTCG30150.029.77141441
TCCAGTC31300.029.51946325
GAGCACA32900.028.485259
CCAGTCA33350.027.57299426
GAGTGGA33550.027.54123334
Produced by FastQC (version 0.11.2)