FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs21_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs21_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7862483
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG6100.016.5902633
GCACACG8000.012.92449911
CGTACGT8950.012.53654534
GATCGGA9700.011.1176381
CACGTCT10800.010.38848614
CACACGT10400.010.36498312
ACACGTC10500.010.2662713
ACGTCTG11150.09.86508915
CGTCTGA11850.09.09669516
CAGTCAC13500.08.79965927
GTACGTA13000.08.63098335
AGCACAC13800.08.1301210
GTCACCG15150.07.84128129
AGTCACC16900.07.810348528
GAGCACA15000.07.77303279
ACCGTAC14200.07.746277332
CCAGTCA15200.07.23656226
TCCAGTC14950.07.063271525
AGAGCAC16600.07.0238248
GAACTCC16950.06.878789421
Produced by FastQC (version 0.11.2)