FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs20_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs20_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7100643
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG6500.010.4918811
CGTTTCG7900.08.63292433
AGTCACG6950.08.54641328
CACACGT8400.07.85682212
CACGTCT10100.07.62345114
ACACGTC8751.8189894E-127.291131513
ACGTCTG8751.8189894E-127.291131515
GTCACGT7904.7293724E-117.24021129
TGGGGGG6401.445369E-87.21942145
GATCGGA8901.8189894E-127.1711761
GTTTCGG10151.8189894E-126.71926934
CGTCTGA10002.7102942E-106.159748616
CCAGTCA12950.06.115623526
TCCAGTC11557.2759576E-126.095032725
AGCACAC11055.16593E-105.773520510
CAGTCAC10801.7844286E-95.70351127
GAGCACA12654.8748916E-105.3910849
AGAGCAC13102.2009772E-105.37382658
Produced by FastQC (version 0.11.2)