FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs1_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs1_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7656136
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC159160.20788554435292164TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG25600.032.5689711
GATCGGA26500.031.0607591
ACGTCTG27250.030.91982715
ACACGTC27450.030.77468513
CACACGT27500.030.63873512
CCGATGT27300.030.300533
CACGTCT27850.030.09570514
CAGTCAC29550.028.73654727
CGTCTGA29600.028.53936216
TATCTCG27300.028.36661139
AGCACAC30300.027.80743410
ACCGATG30900.027.26819432
GTCACCG30850.027.24036829
CGATGTA30700.027.0880734
CACCGAT31400.026.76357731
CGTATGC30200.026.51563544
GAGCACA32000.026.398919
CCAGTCA32000.026.3989126
TCCAGTC32100.026.38520225
TCGGAAG32300.026.2264543
Produced by FastQC (version 0.11.2)