FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs19_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs19_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7345697
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTGG11300.021.22042531
GCACACG11300.021.22013511
ACGTGGC11900.020.3356332
AGTCACG12100.019.9989628
GATCGGA12550.019.6420731
CACACGT13100.018.80818212
GTCACGT13200.018.66569729
ACGTCTG13550.018.3459115
ACACGTC13450.018.31874813
CGTGGCC13000.018.27709233
CACGTCT14350.017.01653714
CGTCTGA15150.016.8440116
TCACGTG14950.016.3337130
TATCTCG15550.015.42115541
CAGTCAC16100.015.30355327
GTGGCCT15350.015.19263434
GAGCACA16450.015.1118829
AGCACAC16550.014.75451910
TCCAGTC17250.014.66600425
GGCCTTA16250.014.21609736
Produced by FastQC (version 0.11.2)