FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs18_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs18_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6446709
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCT5000.013.63978931
GTCACGG5550.013.0803729
CGGCTAC6300.011.52398933
TCACGGC5750.011.47763830
GCACACG7200.09.77724711
GATCGGA7250.09.7144891
AGTCACG7400.09.51299728
ACGGCTA7450.08.56375832
ACACGTC8150.08.36764413
CATCTCG7450.08.26871339
CGTCTGA8600.08.18560216
CACACGT8900.08.1568612
CGTATGC8900.07.909744344
ACGTCTG8450.07.81022715
CACGTCT8400.07.594825714
GGCTACA10400.06.980931334
AGCACAC10000.06.819629710
CAGTCAC11350.06.78382527
GAGCACA13101.8189894E-125.87754349
GCTACAT14300.05.846335435
Produced by FastQC (version 0.11.2)