FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs17_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs17_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5842235
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG7400.013.08046111
ACACGTC8350.012.38264713
GATCGGA8250.012.2715451
CACGTCT8550.012.09299414
ACGTCTG8350.011.85572515
CGTCTGA8600.011.51108216
CACTAGC9050.011.1817931
CACACGT9050.011.1817912
CAGTCAC10850.010.13794127
GAGCACA11050.09.9542789
AGCACAC10800.09.36992610
CCAGTCA11300.09.3448526
TCACTAG12550.08.76452430
TCCAGTC11600.08.72387325
CGTATGC11000.08.39960144
TATCTCG10650.08.26280239
GAACTCC12550.08.23879321
TAGCTTA14300.08.15366834
CTAGCTT13250.08.13560233
AGAGCAC13050.08.091578
Produced by FastQC (version 0.11.2)