FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs16_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs16_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10669766
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC206980.19398738454057943TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG30350.033.99534211
AGTCACG31950.032.0863528
GATCGGA31650.031.7762341
CACACGT33050.031.35123612
CGATCAG33050.031.15286333
ACACGTC34050.030.6243213
CACGATC34000.030.41054231
GTCACGA33950.030.26093529
CGTCTGA34650.029.58611316
CACGTCT35200.029.56131214
ACGTCTG35000.029.35310715
AGCACAC36300.028.60491410
GATCTCG34150.028.60435339
GAGCACA38050.027.4628899
CGTATGC38050.027.40507344
CAGTCAC39050.026.30892627
TCCAGTC39650.025.85532825
AGAGCAC40900.025.5492138
TCACGAT40950.025.35673530
GAACTCC41250.025.27910221
Produced by FastQC (version 0.11.2)