FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs15_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs15_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6552043
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG11650.026.24771911
GTCACAC13200.022.99894329
GATCGGA13200.022.507211
ACGTCTG13950.022.39323415
CACACGT14250.021.7674212
CACGTCT14250.021.45866214
CGTCTGA14350.021.30912616
ACACGTC14400.021.08236513
AGCACAC15800.019.3535410
CAGTCAC16300.018.22029927
TCCAGTC16600.018.02354225
GAGCACA17450.017.6496189
AGAGCAC17750.017.2273778
GAACTCC17500.017.22232821
CCAGTCA17450.017.01953526
CGTATGC14450.016.59464644
AGTCACA18300.016.58940128
TCACACT19950.015.32761630
TCGGAAG20150.015.1774493
GTCTGAA21850.014.39750817
Produced by FastQC (version 0.11.2)