FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs14_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs14_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5258769
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC108410.20615090717998832TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC16650.033.0316713
GCACACG17150.032.06864511
CACACGT17150.032.06864512
ACTTAGG16850.031.8580732
CTTAGGC16850.031.8580733
ACGTCTG17550.031.21238715
CGTCTGA18150.030.18057316
CATCTCG16150.030.1071739
GATCGGA18100.030.0305711
CACGTCT18700.029.41055314
CGTATGC18750.028.0415144
CAGTCAC20150.027.40333427
TCCAGTC20300.026.87573825
AGCACAC20650.026.84660510
CTCGTAT18850.026.72568742
CCAGTCA20650.026.10061626
GAGCACA21800.025.53139
TTAGGCA21400.025.18803234
CACTTAG21900.025.11359631
TAGGCAT20600.025.09867935
Produced by FastQC (version 0.11.2)