FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs13_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs13_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5599441
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG8150.025.64311211
GATCGGA10600.020.3475021
CGATCTC9750.019.40588638
CACACGT10900.019.37534512
ACACGTC10800.019.35105113
CGTCTGA11300.018.88417216
ACGTCTG11550.018.47542615
CACGTCT11700.018.05053514
GATCTCG10500.018.0197539
CACGATC10350.017.643236
CGTATGC12500.017.42327944
CAGTCAC12850.016.94871527
CCAGTCA12600.016.93580626
TCCAGTC12700.016.80245425
AGCACAC12600.016.41201810
CATCACG12950.016.30907833
AGTCACA14000.015.71363528
GAACTCC14050.015.65771521
GAGCACA13750.015.5194969
GTCACAT14250.015.12919829
Produced by FastQC (version 0.11.2)