FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs12_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs12_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5355225
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT325470.6077615786451549TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG45200.039.66560711
AGTCACG44600.039.5086928
CACGTGA45100.039.21737331
GTCACGT45400.038.86095429
ACACGTC46350.038.8238413
CACACGT46650.038.4798612
GATCGGA45550.038.3656041
TCACGTG46250.038.19431730
CACGTCT47350.037.86453214
CGTCTGA47600.037.61944216
GATCTCG42350.037.61009241
ACGTCTG47450.037.5992815
CCAGTCA48150.036.8242326
AGCACAC48750.036.8222610
CAGTCAC48250.036.79350327
CGATCTC42700.036.7886640
GAGCACA49200.036.664679
GAACTCC48800.036.5591421
TCCAGTC48650.036.3101225
AGAGCAC49800.036.178758
Produced by FastQC (version 0.11.2)