FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs11_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs11_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6507088
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC7450.026.28132233
ACGTCCG7750.025.2639832
GTCCGCA9400.020.82966834
CACGTCC10000.019.79942931
AGTCACG10150.019.29008328
GATCGGA11500.018.3704761
CACACGT11800.018.08422712
GCACACG11200.018.07090811
ACGTCTG11600.018.01672215
CCGCACA10600.017.84922836
GTCACGT11300.017.71630329
ACACGTC11850.017.45097513
CGTCTGA12350.016.74445716
CACGTCT12450.016.43326214
TCACGTC12100.016.36316530
TCCGCAC12400.015.96789535
CATCTCG12500.015.31179341
AGCACAC14300.014.922646510
CAGTCAC14400.014.51358227
CGCACAT13700.013.97093737
Produced by FastQC (version 0.11.2)