FastQCFastQC Report
Wed 20 Dec 2017
HW22FAFXX_n01_vs10_171213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22FAFXX_n01_vs10_171213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7202024
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT194860.2705628306709336TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG28300.039.2590935
GTCCCGA27400.037.9794736
CCCGTCC30600.036.73911733
ACCCGTC30450.036.48558432
CACCCGT31250.035.8326531
GCACACG32650.035.57614511
CCGTCCC31300.035.4959934
GATCGGA32550.035.4935231
TCACCCG31750.035.26835630
CCCGATC25750.034.68862538
ACACGTC33850.034.50992613
CACACGT34500.033.85973712
CACGTCT35000.033.37602614
ACGTCTG34850.033.20405215
GTCACCC34100.032.64429529
TCCCGAT29850.032.57738537
CGTCTGA35750.032.42968416
CGATCTC28100.032.25671840
CCGATCT27900.032.2518339
GATCTCG28350.031.89422241
Produced by FastQC (version 0.11.2)