FastQCFastQC Report
Tue 15 Aug 2023
HW22CDRX2_l01_n02_scN1update.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22CDRX2_l01_n02_scN1update.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length90
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTCTTAAAACTCTAAAACAGTTGTACTGCTTCCAAGCAGCAGTATTT15.88235294117647No Hit
AAGCAGTGGTAGCAACGCAGAGTACATGGGCGGCGCGCGGCGGCGGCCGC15.88235294117647Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACCACCACAAGTGGGTGCCCCCGCCCGAGCCCGTGCCGCCGCACATGGCG15.88235294117647No Hit
TGAGGGCTTCAGCGAGGCCCTGAAGCAGTGCAAGTACTCCCCTGGTCTGA15.88235294117647No Hit
AGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATT15.88235294117647No Hit
CCACACCCTACCCGGGGGGGTAGTGTCGAAAAATTAAAATACAGGAGTCT15.88235294117647No Hit
TGAAGTTAAGTAGCTGAATTTAAATTTGTGTTCTCAAATATTTGCTATGA15.88235294117647No Hit
CTCCCGCCCACGCCCCGCTCCCCGCCCCCGGAGCCCCGCGGACGCTACGC15.88235294117647No Hit
TGCACTGCCACCCTGGGCAACTTCGCCAAGGCCACCTGTGATGCCATTTC15.88235294117647No Hit
GCAGTGGTATCAACGCAGAGTACATGGGCGGCGCGCGGCGGCGGCGGCGG15.88235294117647Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTAAGATACAAAACTTAGCTGGGCGGGGTGGCCCCTGCCGGTAATCCCAG15.88235294117647No Hit
ATATGGGGGACGTCCGGGGGCCTTGGTGTTGCTCAGGGCGGGGGTGTTTT15.88235294117647No Hit
CTCGACCATGATAAGTGTGGCCCGTGCTTGGGGCATAGAGGGGCTCATTG15.88235294117647No Hit
CGCCGTCCCCTCGGGGCGGGGGGGGCAACTAGCGTGGTAACTGGCTGGGT15.88235294117647No Hit
GGCGGGCGGTCCGCCGGGAGGCGAGCCTCCGCCCGTCCCCGCCGCGGGCC15.88235294117647No Hit
CATGTACCTGGAGCAGCAAGTGCAATTTTACGAAACGATTGCAGAAAAGC15.88235294117647No Hit
AATTGAAGCAAGGGCAGCCAGTTATGGCGAGGAGTGTTGTGTCATTGATG15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)