FastQCFastQC Report
Tue 29 Oct 2019
HVYWMAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYWMAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18243461
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5227902.865629498700932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCAC13500.027.4841041
GATCCGG9050.026.5283661
GATCCTC15500.025.3458731
GATCACG10200.024.9639361
GATCACC12450.024.5428561
GATCCAG12800.024.4401381
GATCCGC7450.024.4134371
GATCCTG12600.023.0958861
GTCGCCC9150.022.9777145
GATCTGC12450.022.7897951
GATCGTG12800.022.7350121
GATCCGA13150.022.6831461
GATCAAC26300.022.4065231
GATCCGT9200.022.1419261
GATCTGT14600.021.9252721
GATCGGT11650.021.2323551
GATCATG19000.021.0598011
GATCCCA11800.020.9624521
GATCCCG7350.020.7862971
ACGAGCC11300.020.530577145
Produced by FastQC (version 0.11.5)