FastQCFastQC Report
Tue 29 Oct 2019
HVYWMAFXY_n02_DJ52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYWMAFXY_n02_DJ52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85142990
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC234950.033.796831
GATCGCG165900.033.2640841
GATCACG349850.032.480631
GATCAAC917100.030.7114161
GATCCGG277450.030.3709741
GATCCAG526950.030.275451
GATCGTG414200.029.7804681
GATCCAC562150.029.3213881
GATCCTG444300.028.6769351
GATCCCG271950.028.5319861
GATCGCC260050.027.8019711
GATCAGC490600.026.5322571
GATCCGA488350.025.9268861
GATCACC429300.025.827591
GATCTGC425050.025.7616831
GATCATG660050.025.510721
GATCGCA345150.025.2121961
GATCCCA449800.024.8923071
GATCGGC347550.023.9322451
GATCCGT368750.023.539621