FastQCFastQC Report
Tue 29 Oct 2019
HVYWMAFXY_n02_DJ51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYWMAFXY_n02_DJ51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57770956
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGG174250.032.1694831
GATCCGC147300.031.6060121
GATCACG232600.029.5553461
GATCGCG102050.028.6371271
GATCCCG171650.028.0941071
GATCCAG347650.027.6591421
GATCCTG300600.027.4048081
GATCGTG283800.027.238441
GATCTGC272950.026.4348831
GATCAGC319400.025.9733831
GATCCAC358250.025.403581
GATCCGT241800.025.1018961
GATCGCC165850.024.7041761
GATCCGA323700.024.6874521
GATCAAC611800.024.3223361
GATCACC274700.024.1018051
GATCGCA237700.023.5823841
GATCGGC217600.023.39461
GATCTGG285700.022.7677251
GATCCTC393850.022.7575241