FastQCFastQC Report
Tue 29 Oct 2019
HVYWMAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYWMAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18243461
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG16150.031.6452121
GATCCAG18300.031.4674151
GATCCGC10800.031.3253771
GATCCGG8800.030.2650381
GATCCCG9450.029.706741
GATCCAC17950.029.27391
GTCGCCC11500.029.136309145
GATCTGC15050.028.6970271
GATCACG12050.028.0758531
ACGGTCC7050.027.896463145
GATCCGA16500.027.4839251
GATCGTG15400.025.7077521
GATCCGT11750.025.1170231
AAGGCCC14050.024.885103145
GATCATG22200.024.6423871
GATCAAC31350.024.1087071
GATCTAC32800.023.7012941
CGCATGC13900.023.057508145
GATCAAG30950.023.0248451
GATCAGC18500.022.956331
Produced by FastQC (version 0.11.5)