FastQCFastQC Report
Tue 29 Oct 2019
HVYWMAFXY_n01_DJ52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYWMAFXY_n01_DJ52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85142990
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATAACCATCTCGTAT1301240.15282996286599754TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC233300.033.4065671
GATCGCG152250.031.5238191
GATCACG343850.030.256661
GATCCGG281750.029.2573261
GATCGTG404000.028.533421
GATCAAC873750.027.962811
GATCGGA1087900.027.8697551
GATCCAG521800.027.7884311
GATCCAC563700.027.613541
GATCCTG438450.026.1095961
GATCTGC422400.025.8487261
GATCAGC486500.025.6767771
GATCGCC261600.025.5523781
GATCCCG268300.025.454721
GATCCGA488850.024.7228241
GATCATG650500.023.9289051
GATCGCA340900.023.1174891
GATCCGT367400.022.9892521
GATCACC408100.022.881651
GATCCCA447500.022.2279381
Produced by FastQC (version 0.11.5)