FastQCFastQC Report
Tue 29 Oct 2019
HVYWMAFXY_n01_DJ51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYWMAFXY_n01_DJ51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57770956
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC150550.033.5169261
GATCCGG170450.031.602941
GATCGCG99650.029.9019991
GATCACG225000.027.5498521
GATCCCG170150.026.3325061
GATCCAG322300.026.2510221
GATCCTG289050.025.5084271
GATCGTG272650.024.3836961
GATCTGC269800.024.1307091
GATCCAC370650.024.0002771
GATCCGT229200.023.9451161
GATCCGA322550.023.938021
GATCAGC307000.023.2612761
GATCAAC609450.023.1494391
GATCGCC158650.022.940311
GATCCTC396400.022.8618851
GATCGCA235250.021.4802271
GATCGTC348450.021.0559941
GATCTCG288450.020.987061
GATCACC280000.020.9731031