FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16157398
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206972012.809735825038166No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1199240.7422234693977334Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG215410.13331973378386794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGA265250.061.5318952
GCACACG125700.061.4508418
AGAGCAC154100.059.16348615
AGCACAC129000.058.25496317
GCACGCG23500.056.8411518
ACTGAAG286900.056.7858434
AGATCAT243550.055.6555259
CACACGT91800.055.1552119
GTGACTG105050.054.5418661
CACGCGT15350.054.44401619
GATCATC113300.053.53838310
CTGAAGA325600.053.2684175
TGACTGG110800.053.2387852
ATAACTG312300.052.8245931
TTAGCTA79600.052.40684519
TCATACT108250.051.8826712
ATCATCC113100.051.59609611
CACACGG20150.051.53971519
CGCGCGT25900.051.16819419
ACTGGAG124350.051.1268844
Produced by FastQC (version 0.11.5)