FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11746752
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1342571.1429287006314597No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1186291.009887669374479Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG210350.036.0900466
AAGGGGG212350.035.98072467
CGCCGTA324800.032.8868447
AGGGGGG244350.032.54351468
TCGCCGT328800.032.32681746
CCGTATC328700.032.03865449
GGTCGCC339000.031.76765344
GCCGTAT337900.031.70493948
GTCGCCG339800.031.70370345
CGTATCA339650.031.10920150
GTGGTCG351000.030.5917142
TGGTCGC351350.030.48141543
CTCGGTG346550.029.8728538
GGTGGTC366800.029.39800641
ATCTCGG354450.029.1386736
TCTCGGT357150.028.95770637
GATCTCG355000.028.7680635
TCGGTGG371800.028.6541239
AAAAGGG268800.028.4638165
CGGTGGT383250.028.32758740
Produced by FastQC (version 0.11.5)