FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_1922.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_1922.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10326263
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC920250.891174280569844Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG749200.7255286835130966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCGC10350.030.4803141
CGCCGTA272950.029.65891647
AAAGGGG187700.029.59236166
TCGCCGT276600.029.00167346
GGTCGCC283450.028.78166644
CCGTATC276050.028.55217449
CGGCGCT23100.028.5274661
GTCGCCG284100.028.47026345
GGGGGAT30700.028.3159141
AAGGGGG197150.028.26253567
GCCGTAT286600.028.1120148
GTGGTCG296700.027.55491842
CGTATCA289100.027.4208450
GCACACG25650.027.2885318
CTCGGTG292300.026.76078438
AGAGCAC52000.026.584815
AGGGGGG214200.026.3072568
GGTGGTC313500.026.290441
TGGTCGC312250.026.13785443
ATCTCGG300900.026.0776136
Produced by FastQC (version 0.11.5)